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THP-1 Cells Gr= owth Media

Tuesday, January 24, 2006

3:25 PM

MEDIA TYPE                        =                 VOLUME
RPMI                   =                           42.45 mL

 

FBS                        =                         5.0 mL

 

1M HEPES                  Â=  Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â  0.5 mL

5000 U/mL Penicillin + 5 mg/mL Streptomycin  =       0.5 mL

200mM Glutamine                Â=  Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â  0.5 mL

250mg/mL Glucose                 Â=  Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â  0.5 mL

100 mM Sodium Pyruvate            =                  0.5 mL

 

50 mM beta-Mercaptoethanol         Â=  Â Â Â Â Â Â Â Â Â Â Â Â Â  0.05 mL

 

                        =                   TOTAL  50.00 mL

 

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