R0

Differentiation (Made Mediator

ML Differentiation Media

rom SM 2/6/6 wells 1-3

To SM 2/8 6 well

From SM 2/8/6 Wells 1-3 (Norma)

To NO PIMA SM 2/6/6 2 mL tubes

took 10 ml of CMV-FWB (CGCAAATGGGCGGTAGGC)

2x 1% Agarose gel RR minus chetrum bromide

Made 2% Agarose gel their

100 ML per Mix try

Made 2.5% Agarose 6d EPROM

Quantification (compare to pg 20)

Made differentiation Media Epact

Made loom PER Mix spots/

Made 2.5% Agarosc Gel tp's

Made 100 Ml PER Mix try. *Tag may have been low

To 6 mL Differentiation Media

Made 2.55% Agarose gel PR3

SM 2/20/6 AO Well Plates

Made 10m per Mix PR5 2 MM Mgll2

Made 2.5% Agarose Gel pR34

To 2 mL tubes SM 2/20/610 PMA

Made 2x Growth Media forth HP-I celestial

Made 100 ML BCR Mix PR5 *reason

Made 2.5% Agarose Gel -hoping

Made 140m, PCR Mix preset

Made 2.5% Agarose Gel it PR3

Made 140 ML Per Mix trail

Made 2.5% Agarose Gel tapir

Made 140 ML BCR Mix LIAR 5

Made 2.5% Agarose Gel EARTH

Made 0.8% Agarose Get Lepers

Made 0.8% Agarose Gel PR3 (5prl Et-Br)

Made 280m PCB Mix PR5

Made 2x 2% Agarose Gel PRI

Plated I GAY Sto on sops plate w/

5 colonies on SOB plate

From SM 1 tube in shaker AM shaker too long

From sorb plate (3/14/6) picked colonies I +2

3/21 SM & 51M? isolated DNA PRF

Made 0.8% Agaros@GetsPR31

From SOB plate 3/14/6 picked 425 colonies

From SM40 SM 50

Made 1% Agarose Gel EBOR

Plated I GAY Sto on sops plate w/

Picked from 4/3/6 sops Plate

Plasmid Isolation from [silo) (548 L

Made 1% Agarose Gel PXs

Made 140 are PER Mix PR5

Made 2% Agarose Gel p

Made 1% Agarose Gel LARS

Made 140 ML PER Mix tpR51

Made 2% Agarosc Gel

Transferred Ex-AA Ex-IB to 2 mL tubes

FEE-I Quantification

LExt Made 2% Agarose gel prêt

Ea Quantification

Litter Earl per

_ txt & tEx-21 Made 2% Agarose Gel tears

Exit-Zs Made 2% Agarose Gel PER

LI-EX. Made 2% Agarose Gel LARS

Ex-2 Made 2% Agarose Gel *APRS

EX-2 Made 2% Agarose Get HAAS

_ Lt Ex-2 Made 2% Agarose Gel #